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SMTP RELAY in MTA mode failed, error code defer (110) connection time out

today I replace the barracuda email protection by the Sophos XG as anti-spam proxy in network,  I follow the configuration by this link https://community.sophos.com/kb/en-us/123522,  unfortunately the migration is failed,  the user cannot send out or receive the email,    I saw all email in the mail pool of Sophos,    but all the status is fail in both direction (in and out),   the failed error code is defer 110  ,   I am confused about this error,  because I can reach internal exchange server from sophos,   and I can also reach port 25 from internet to public IP (which is statice NAT to Sophos private IP).   who can help me analysis this problem,  it is critical problem .



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